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depolarization or desensitization of the AChR channel Postsynaptic DNMT occur as a result of a reduced number or reduced reactivity of receptors The former may result from the complement-mediated destruction of receptor sites in MG The latter may result from hereditary AChR subunit mutations, which may affect the normal mechanics of channel opening and closing Regardless of the cause, the nal common pathway is a reduction of the muscle EPP amplitude to the extent that NMT fails in one or many muscle bers The bulbous nerve terminal of the presynaptic region contains a number of subcellular components, the most notable of which are the multiple synaptic vesicles containing the neurotransmitter acetylcholine (ACh) (Fig 23 6) Presynaptic function can be summarized by a number of sequential steps These include 1 packaging of ACh into synaptic vesicles; 2 clustering of synaptic vesicles in the vicinity of the active zone; 3 docking of the synaptic vesicle at the active zone; 4 calcium-triggered fusion pore opening with exocytotic release of ACh into the synaptic cleft; 5 endocytosis and re lling of vesicles with migration back to the active or mobilization sites The synaptic vesicles tend to cluster close to the presynaptic membrane in small groups in membranedense regions referred to as active zones418,440 442 Although there are non-calcium-dependent mechanisms of ACh release, the ef cient function of these active zones is dependent on calcium entry into the distal motor nerve terminal The P/Q type calcium channels integral to this process are distributed along the active zones at the sites of vesicle fusion on the presynaptic membrane Calcium in ux through these channels greatly facilitates vesicle fusion and subsequent exocytotic release of the AChcontaining vesicles known as quanta into the synaptic cleft In mammalian systems, 50 300 vesicles are typically released in response to a single nerve action potential, referred to in the aggregate as quantal content1 It is estimated that 75 100 microseconds are required between arrival of the nerve action potential at the nerve terminal and quantal release357,443,444 There are approximately 200 400 103 individual synaptic vesicles contained in the average nerve terminal In mammalian NMJs, approximately 20% of these are positioned for immediate release in the active zones445,446 Within each vesicle, there are between 5 103 and 10 103 molecules of ACh447 452 The mobilization pool or reserve pool is estimated to contain 300 103 vesicles that can be moved readily to the active zone region450,453 Following vesicle fusion with the presynaptic membrane and exocytosis, ACh resynthesis and repackaging (endocytosis) take place Experimental data suggest that the rate of resynthesis parallels the rate of ACh release under normal physiological conditions.

Application packages can be installed onto SymbianOS devices through several vectors namely MMS, Bluetooth, HTTP/S, IRdA, and USB tethering No matter the route, all installation is handled through the SWInstall process SWInstall acts as the gatekeeper for a SymbianOS device It has sufficient capabilities to access the necessary file system paths It validates all installation packages to ensure that they meet the signing requirements for the requested capabilities, that the binaries within the package have not been modified, and that the installer is not overwriting an existing application Validation occurs in several steps: To begin, SWInstall identifies the files to be installed (remembering that a single installation package can include binaries for multiple platforms) Then it enumerates the capabilities requested by the listed executable images (DLLs and EXEs) SWInstall proceeds to chain the signatures on the SIS file back to trusted SymbianOS code-signing CAs generally Symbian Signed or a device manufacturer Each CA may only certify particular capabilities If the set of requested capabilities is not a subset of the certifiable capabilities, then one of two things occurs If the requested capabilities are system level or higher, the installation will fail If the requested capabilities are user level, the device owner will be asked whether installation should continue SWInstall also ensures that the SID is unique on the phone, that installers with protected range SIDs are appropriately signed, and that a Vendor ID is only specified with signed applications After validation, SWInstall copies the contained resources to the required destinations Binaries are placed in \sys\bin, resources are written to \resource, and the associated private directories are determined from an applications SID The installer will also write files into the import directory of another executable (\private\<SID>\import) to allow for delivering data to other applications Installing into this import directory must happen at installation; most applications will not have the necessary capabilities to write to this directory When applications are installed to removable media, the SWInstall process will record the SHA-1 hash of the binaries to a private location on internal media This prevents an individual from installing an application, removing the media, and modifying the binary to increase capabilities Remember that capabilities are only checked against the signature at install time.

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Device Manager Select the Hardware tab and click the Device Manager button Note the list of hardware installed in your system Expand various items to see the list of devices in each area Highlighting any device, right-clicking, and selecting Properties will give you con guration information for that device You ll return here later in the lab manual to con gure these devices

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