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+ Test chemical in solvent or solvent alone + Culture of S. typhimurium tester strain S9 + S9 (9,000 g supernatant) fraction from PCB-induced rat liver

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1996, 7, 115 127 143 Xiong, X; Smith, J L; Chen, M S Effect of incorporation of cidofovir into DNA by human cytomegalovirus DNA polymerase on DNA elongation Antimicrob Agents Chemother 1997, 41, 594 599 144 De Clercq, E Towards an effective chemotherapy of virus infections: therapeutic potential of cidofovir [(S)-1-[3-hydroxy-2-(phosphonomethoxy)propyl]cytosine, HPMPC] for the treatment of DNA virus infections Collect Czech Chem Commun 1998, 63, 480 506 145 Naesens, L; Snoeck, R; Andrei, G; Balzarini, J; Neyts, J; De Clercq, E HPMPC (cidofovir), PMEA (adefovir) and related acyclic nucleoside phosphonate analogues: a review of their pharmacology and clinical potential in the treatment of viral infections Antiviral Chem Chemother 1997, 8, 1 23 146 Cundy, K C Clinical pharmacokinetics of the antiviral nucleotide analogues cidofovir and adefovir Clin Pharmacokinet 1999, 36, 127 143 147 De Clercq, E.

Evaluate results (# of revertant colonies/plate)

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Let Pjk t be the probability of being in level k at time t conditional on being in ^ level j in the steady state at time 0. Let Pjk s be its Laplace transform. Let mj t be ^ the conditional steady-state mean given level j at time 0 and let mj s be its Laplace transform. Clearly, mj t

^ Pjk s bk :

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In brief, the test is carried out (Figure 21.5) by mixing a suspension of bacterial cells with molten top agar. This also contains cofactors, S-9 fraction, and the material to be tested. The mixture is poured onto Petri plates containing hardened minimal agar. The number of bacteria that revert and acquire the wild-type ability to grow in the absence of histidine can be estimated by counting the colonies that develop on incubation. To provide a valid test, a number of concentrations are tested, and positive controls with known mutagens are included along with negative controls that lack only the test compound. The entire test is replicated often enough to satisfy appropriate statistical tests for signi cance. Parallel tests without the S-9 fraction may help distinguish between chemicals with intrinsic mutagenic potential and those that require metabolic activation. The question of correlation between mutagenicity and carcinogenicity is crucial in any consideration of the utility of this or similar tests. In general, this appears to be high, although a small proportion of both false positives and false negatives occurs. For example, certain base analogues and inorganics such as manganese are not carcinogens but are mutagens in the Ames test, whereas diethylstilbestrol (DES) is a carcinogen but not a bacterial mutagen (see 12 for additional detail).

Therapeutic potential of HPMPC as an antiviral drug Rev Med Virol 1993, 3, 85 96 148 Kern, E R; Hartline, C; Harden, E; Keith, K; Rodriguez, N; Beadle, J R; Hostetler, K Y Enhanced inhibition of orthopoxvirus replication in vitro by alkoxyalkyl esters of cidofovir and cyclic cidofovir Antimicrob Agents Chemother 2002, 46, 991 995 149 Keith, K A; Hitchcock, M J M; Lee, W A; Holy, A; Kern, E R Evaluation of nucleoside phosphonates and their analogs and prodrugs for inhibition of orthopoxvirus replication Antimicrob Agents Chemother 2003, 47, 2193 2198..

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Related Tests. Related tests include tests based on reverse mutations, as in the Ames test, as well as tests based on forward mutations. Examples include:

Theorem 17.6.1. Assume that the level-holding-time cdf depends only on the originating level, that is, Fjk t Gj t . The steady-state transition probabilities conditional on the level for a single SMP source have the matrix of Laplace transforms ^ ^ ^ ^ P s De s ge s P sj0 ; ^ ^ where P sj0 is the matrix in Eq. (17.14), ge s is the matrix with elements ^ ^ gejk s Pjk gje s Pjk ^ 1 gj s ; sm Gj 17:36 17:35

150 Buller, R M; Owens, G; Schriewer, J; Melman, L; Beadle, J R; Hostetler, K Y Ef cacy of oral active ether lipid analogs of cidofovir in a lethal mousepox model Virology 2004, 318, 474 481 151 Smee, D F; Sidwell, R W A review of compounds exhibiting anti-orthopoxvirus activity in animal models Antiviral Res 2003, 57, 41 52 152 Neyts, J; De Clercq, E Ef cacy of (S)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine for the treatment of lethal vaccinia virus-infections in severe combined immune-de ciency (SCID) mice J Med Virol 1993, 41, 242 246 153 Bray, M; Martinez, M; Smee, D F; Kefauver, D; Thompson, E; Huggins, J W Cidofovir protects mice against lethal aerosol or intranasal cowpox virus challenge J Infect Dis 2000, 181, 10 19 154 Bray, M; Martinez, M; Kefauver, D; West, M; Roy, C.

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